FastQCFastQC Report
Thu 2 Jun 2016
DRR028380_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028380_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830193
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53330.2913900337286833No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17000.052.4291154
ATCAACG18450.047.9146043
CAACGCA19550.046.334395
GTATCAA20300.045.34521
AACGCAG20500.043.1266946
GGTATCA12800.037.3729671
GTACATG38100.036.1453361
TATCAAC24650.035.8689652
ACGCAGA23700.035.7777867
CGCAGAG23250.035.53618
GACGACC14550.029.887396145
TACATGG46300.028.0187032
ACCGTAT1801.5245256E-424.1583148
ACATGGG52500.023.463573
GTCTCGC4950.023.4281731
CATGGGG42750.022.713554
TGTACCG2252.6624391E-522.5452865
GCAGAGT36150.021.853359
ATGGGGG28700.021.209855
TCTGTCG6000.020.5345678
Produced by FastQC (version 0.11.5)