FastQCFastQC Report
Thu 2 Jun 2016
DRR028380_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028380_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830193
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30020.16402641688608796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26780.1463233658963836No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23050.082.125881
GTATCAA45550.063.691581
ATCAACG50650.056.2345243
TCAACGC50800.055.7831464
CAACGCA52350.053.601155
AACGCAG54450.051.8002136
TATCAAC57100.049.7553672
ACGCAGA62150.044.9158337
CGCAGAG62050.044.6376578
GCAGAGT79600.034.249539
GTACATG35850.027.514411
AACGCCG1500.001493908124.1689936
TACATGG41700.024.1584282
CAGAGTA73300.022.4544169
ACATGGG44000.021.9073053
CATGGGG39850.020.3694674
CCGTGCA2908.248762E-620.0019269
GCGTTAT1850.00503686919.6023751
TATACTG16550.019.276785
TCTGTCG5707.2759576E-1219.0807868
Produced by FastQC (version 0.11.5)