FastQCFastQC Report
Thu 2 Jun 2016
DRR028379_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028379_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923381
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57960.30134435143115174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22800.051.8006634
ATCAACG23650.050.246593
CAACGCA23950.049.3185125
AACGCAG25250.046.778126
GTATCAA27800.046.1461681
GGTATCA18200.042.2125551
GTACATG49200.040.658481
ACGCAGA29100.040.5913777
TACATGG49100.038.3764722
TATCAAC32100.038.1552962
CGCAGAG29800.037.207098
ACATGGG56350.032.0183753
CATGGGG48150.030.848934
GCAGAGT37850.026.6161219
GACGACC16000.025.366693145
ATGGGGG36100.022.6829265
GAGTACA35500.022.2538591
GTATAAT18650.018.6538981
AGTACAT38000.018.4995562
GTATTAG15000.018.3611341
Produced by FastQC (version 0.11.5)