FastQCFastQC Report
Thu 2 Jun 2016
DRR028379_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028379_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923381
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36640.19049787847545546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30930.16081057263225537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29050.079.131941
GTATCAA53950.062.0996671
TCAACGC61800.053.8301734
ATCAACG61950.053.5828443
CAACGCA63550.052.0219155
AACGCAG66150.050.0868156
TATCAAC68250.048.4243472
ACGCAGA74850.043.877667
CGCAGAG75150.043.5095528
GCAGAGT86450.036.9837279
GTACATG47000.033.1725851
TACATGG49450.031.2187062
ACATGGG50750.030.2762033
CATGGGG43000.028.1481324
CAGAGTA85800.024.504619
GTGGTAT16650.021.3412841
ATGGGGG32400.021.2576715
ACCGCGT2155.05854E-420.2325158
TGGTATC16050.019.8691462
AGAGTAC81100.018.94034610-14
Produced by FastQC (version 0.11.5)