FastQCFastQC Report
Thu 2 Jun 2016
DRR028378_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028378_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989839
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28120.284086603983072No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC11400.054.0279664
GGTATCA7700.053.6508671
GTATCAA12950.052.0482061
ATCAACG11650.051.6219863
GTACATG29850.043.461161
TATCAAC14750.043.2309882
CAACGCA13750.043.2153175
AACGCAG13550.042.24886
TACATGG28900.039.8661042
ACGCAGA15450.037.055037
ACATGGG31300.035.4184533
CGCAGAG16150.032.3078848
CATGGGG27400.031.9992184
ATGGGGG18800.027.7524415
GAGTACA18050.025.296241
GCAGAGT19900.024.034749
GTATTAG4900.023.6655451
GACGACC13050.022.767845145
CTATTAG3554.14002E-922.4572681
TATACTG6850.020.09975
Produced by FastQC (version 0.11.5)