FastQCFastQC Report
Thu 2 Jun 2016
DRR028378_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028378_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989839
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18540.1873031876901193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17880.18063543667202445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10070.10173371629123523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.070.577991
GTATCAA23750.065.957811
ATCAACG28800.055.1115233
TCAACGC28750.054.703194
CAACGCA31200.050.1981165
TATCAAC31350.049.704042
AACGCAG32050.048.8668066
ACGCAGA35750.042.9979827
CGCAGAG37800.040.4742628
GTACATG29950.036.0799141
ACATGGG29650.035.443293
TACATGG30600.034.5797772
GCAGAGT43200.034.4079179
CATGGGG27100.029.418074
TATATCG1050.007721177327.6096863
ACCGCGT1606.8226895E-527.1906458
CCTAGAC4300.026.96763
ATGGGGG16400.025.2010865
CAGAGTA43450.024.531039
GTGGTAT6900.022.0722331
Produced by FastQC (version 0.11.5)