FastQCFastQC Report
Thu 2 Jun 2016
DRR028377_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028377_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574626
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44290.28127314041556534No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19150.055.9994854
ATCAACG20050.053.8437543
CAACGCA20850.051.7827535
AACGCAG21550.050.4353566
GTATCAA24650.045.8752821
GGTATCA13400.044.8997961
ACGCAGA25000.042.6098447
TATCAAC27750.039.4355662
CGCAGAG26550.039.30728
GTACATG40750.036.6446571
TACATGG43900.033.0171552
ACATGGG46400.029.3564323
GCAGAGT34700.027.7794729
CATGGGG38300.026.8646184
GACGACC14750.024.075659145
GAGTACA31200.020.9102381
TGTACCG2155.076233E-420.22175
AGTACAT33800.018.4397872
ATGGGGG30750.018.3803775
GTATAGC5001.10812834E-817.3973181
Produced by FastQC (version 0.11.5)