FastQCFastQC Report
Thu 2 Jun 2016
DRR028377_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028377_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574626
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38460.24424847551101025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32210.20455651056187313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17680.11228063044811912No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23600.074.999531
GTATCAA48350.068.71471
TCAACGC53650.060.6536834
ATCAACG54450.060.028743
CAACGCA55800.058.0735055
AACGCAG58100.055.7745486
TATCAAC60100.054.264852
ACGCAGA64300.050.5093427
CGCAGAG67100.048.5096938
GCAGAGT77500.040.596899
GTACATG41750.037.5299451
ACATGGG44450.034.40263
TACATGG45400.033.8423542
CATGGGG35250.028.989494
TGGTATC11700.026.6356412
GTGGTAT13150.025.9270321
CAGAGTA75900.023.7827639
GAGTACA31550.020.693021
ATGGGGG28450.019.1110125
TCTATAC10400.018.8152923
Produced by FastQC (version 0.11.5)