FastQCFastQC Report
Thu 2 Jun 2016
DRR028376_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028376_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880156
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24930.28324524288876063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8200.054.803981
ATCAACG11050.047.2204633
GTATCAA12300.047.1432151
TCAACGC11550.045.173714
GTACATG25700.041.7410051
AACGCAG13300.039.7769246
CAACGCA13600.038.8994945
TACATGG26300.037.4751322
ACGCAGA15050.035.153717
TATCAAC16050.034.316152
CGCAGAG15650.034.2690548
ACATGGG28450.032.6052633
CATGGGG24250.027.1934854
GAGTACA16900.026.1623921
GACGACC12300.025.336586145
GCAGAGT20350.024.2174979
ATGGGGG18050.022.0823615
GTACTAA4251.4733814E-920.4657021
AGTACAT20600.020.0524582
GTATAGA6250.019.7152921
Produced by FastQC (version 0.11.5)