FastQCFastQC Report
Thu 2 Jun 2016
DRR028376_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028376_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880156
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21370.24279786765073466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19980.22700521271229193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10810.12281913660760137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14500.076.521081
GTATCAA26550.065.008491
TAGACCG400.00605085154.3745355
ATCAACG32900.051.990863
TCAACGC33150.051.598774
ATACGAC858.9241075E-751.176036
CAACGCA34550.049.5220075
AACGCAG36350.047.468646
TATCAAC36700.046.607612
ACGCAGA40900.041.833387
CGCAGAG42100.041.15768
GCAGAGT46850.036.2110029
GTACATG23600.031.650671
TACATGG24200.030.2494092
ACATGGG26600.027.7926163
CAGAGTA44550.026.6888839
CATGGGG23350.025.1425324
GAGTACA17100.024.1733261
CTACTAC3203.374589E-822.6624951
AGACCGC1800.00430688320.1387166
Produced by FastQC (version 0.11.5)