FastQCFastQC Report
Thu 2 Jun 2016
DRR028375_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028375_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641853
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47140.2871146198837533No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13100.050.901951
TCAACGC19550.050.0363244
ATCAACG20450.047.481363
CAACGCA20750.047.1426545
GTATCAA22150.045.1568071
AACGCAG22900.043.0343326
GTACATG39550.041.7836381
ACGCAGA25550.037.4387447
CGCAGAG25400.036.8039328
TACATGG43900.035.6544882
TATCAAC28150.035.009462
GACGACC13150.029.207987145
ACATGGG52850.027.970283
CATGGGG38750.025.805114
GCAGAGT36300.024.157039
GAGTACA30500.020.436971
CATGGGA17050.019.9743144
GTATACG2050.00914336317.6780381
GTATTAG13700.017.4586751
TATACAG15850.017.3721145
Produced by FastQC (version 0.11.5)