FastQCFastQC Report
Thu 2 Jun 2016
DRR028375_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028375_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641853
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33960.20683946735791814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30910.1882628956429108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16640.10134890273367957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.081.662181
GTATCAA46850.064.7038041
ATCAACG56050.053.6625143
TCAACGC56100.053.4854934
CAACGCA57950.051.5420075
TATCAAC60150.050.0047232
AACGCAG60700.049.445786
ACGCAGA67800.043.6262667
CGCAGAG68100.043.2211658
GTGGTAT12300.035.9656451
GCAGAGT81150.035.6452759
GTACATG39500.033.5982861
TGGTATC11600.032.4895632
TACATGG42050.031.0245172
ACATGGG43050.029.798793
CATGGGG38000.023.459574
AGACCGT2202.2307435E-523.0670956
CAGAGTA77900.022.8936619
CATGGGA15050.019.2629174
GTACGAT2659.3577124E-519.1500436
Produced by FastQC (version 0.11.5)