FastQCFastQC Report
Thu 2 Jun 2016
DRR028374_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028374_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714370
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49870.2908940310434737No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25200.057.2333871
TCAACGC25400.051.0699464
ATCAACG25550.050.770123
GGTATCA15600.048.317631
CAACGCA27300.046.7206425
AACGCAG29050.043.6566776
TATCAAC32550.042.9724922
GTACATG50100.039.348491
ACGCAGA32650.037.957467
CGCAGAG32200.036.2371648
TACATGG51900.034.631282
ACATGGG62850.025.8278853
GCAGAGT40850.025.3711939
CATGGGG49750.024.9085874
GACGACC13700.022.748083145
GAGTACA39300.021.2081221
ACCGCGT2155.07024E-420.2253958
GTATAAG9150.019.0102181
GTATATA14900.018.9703461
AGTACAT40750.018.8522192
Produced by FastQC (version 0.11.5)