FastQCFastQC Report
Thu 2 Jun 2016
DRR028374_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028374_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714370
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40190.2344301405180912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34040.1985569042855393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18970.11065289289943245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29300.079.205251
GTATCAA56300.067.112121
ATCAACG67200.056.19033
TCAACGC67500.056.0479354
CAACGCA70500.053.1690645
AACGCAG73550.051.1613856
TATCAAC74000.050.9289252
ACGCAGA83550.044.864387
CGCAGAG84400.044.5843548
GCAGAGT95300.038.72429
GTACATG50300.031.5753211
TACATGG52650.029.7336522
ACATGGG56150.026.7185863
CATGGGG48400.025.7558544
CAGAGTA92900.025.2083479
TACCGTA1851.8308498E-423.5145727
GTGGTAT13600.023.4631021
TGGTATC14950.020.8458772
TAGCGTC1800.004307181620.1397975
GTACCGT1850.005047003719.5954766
Produced by FastQC (version 0.11.5)