FastQCFastQC Report
Thu 2 Jun 2016
DRR028373_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028373_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874702
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55520.2961537353670077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18900.043.3209344
ATCAACG19550.042.993623
GGTATCA16100.039.6166271
GTATCAA23300.039.5064931
CAACGCA21400.038.263155
AACGCAG22450.035.828016
TATCAAC27800.031.5421662
CGCAGAG26150.030.4839978
GTACATG38350.030.0505491
ACGCAGA26950.029.8479927
TACATGG43200.025.6660232
ACATGGG44600.023.069933
GACGACC12650.021.769857145
GCAGAGT37950.020.0511849
CATGGGG36300.019.9606444
GTATATA24200.019.1683731
GTCTAGC4954.765752E-1019.0352611
GAGTACA33000.018.6691971
TATACCG2000.00794194718.115745
GTATAGA14300.017.7399641
Produced by FastQC (version 0.11.5)