FastQCFastQC Report
Thu 2 Jun 2016
DRR028373_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028373_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874702
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35790.19091034201702456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29370.15666489927465807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24800.074.870421
GTATCAA46200.062.3261831
TCAACGC53950.052.9272274
ATCAACG54550.052.079373
CAACGCA56850.049.733335
TATCAAC58550.048.7689862
AACGCAG59200.047.6366586
ACGCAGA66050.042.4767767
CGCAGAG65550.042.4689878
GCAGAGT79850.033.2291769
GTACATG38750.032.0071071
TACATGG42600.028.240532
AACGCCG1855.8222577E-627.4308766
ACATGGG41850.027.014913
GTACGAC1358.103178E-426.8417363
CGTACAC2001.0692373E-525.3654423
CAGAGTA77300.023.7276349
GCACCGT2802.3461689E-723.3022526
GAGTACA30300.020.8256511
CGTATGG2104.327061E-420.7064822
Produced by FastQC (version 0.11.5)