FastQCFastQC Report
Thu 2 Jun 2016
DRR028372_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028372_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487723
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43710.29380469348124616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18550.054.3047684
ATCAACG19300.052.192723
GTATCAA22000.051.728751
CAACGCA20500.049.139195
AACGCAG21700.046.421826
GTACATG41100.045.5022541
GGTATCA12300.044.198871
TATCAAC26700.041.532
TACATGG42250.040.482472
ACGCAGA24550.039.8546187
CGCAGAG23900.038.8157928
ACATGGG50850.032.9210323
CATGGGG41550.030.0002234
GCAGAGT31550.026.1879739
GTACGGA1750.003667905720.7061356
ATGGGGG35050.020.4698315
CGACGAC5002.5465852E-1120.293383145
TATACTG13450.019.3975685
GAGTACA29700.017.3283391
TAGTACG3352.8694063E-517.306624
Produced by FastQC (version 0.11.5)