FastQCFastQC Report
Thu 2 Jun 2016
DRR028372_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028372_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487723
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31160.20944759205846786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26450.17778847271972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23300.083.423551
GTATCAA45800.064.927431
TCAACGC50600.058.1558344
ATCAACG51450.057.3359223
CAACGCA53000.054.9993745
AACGCAG55250.053.0220686
TATCAAC57350.051.3109932
ACGCAGA63550.045.7547767
CGCAGAG63900.045.2772068
GCAGAGT75000.037.9960869
GTACATG40100.035.269561
TACATGG41650.031.4978832
ACATGGG44850.029.5737533
AACGCCG1050.00770361327.6234726
CATGGGG34100.026.9939964
TAGTGCG1450.001225579225.0040079
GTGGTAT11200.022.6652151
CAGAGTA74000.022.145449
ACCGTCC1900.005883760319.0820038
AGTACCG1950.006835762418.5927225
Produced by FastQC (version 0.11.5)