FastQCFastQC Report
Thu 2 Jun 2016
DRR028371_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028371_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445734
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40890.2828321115779251No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14400.055.3436054
ATCAACG14400.054.840483
GTATCAA17450.050.67371
CAACGCA16700.047.7230875
AACGCAG17100.046.608386
GGTATCA10150.042.131231
TATCAAC21300.040.146272
GTACATG32000.039.4109951
ACGCAGA19800.038.0623747
CGCAGAG19700.037.8877378
TACATGG33400.034.497962
ACATGGG39150.026.2780913
CATGGGG31300.023.841314
GCAGAGT29650.022.9736949
GAGTACA24150.019.5080761
GTATAGA10950.018.5336991
GTATTAG11350.017.8805271
ATAATAC15650.017.5916463
GTATATA15900.017.3222641
AGAGTAC23650.016.5430265
Produced by FastQC (version 0.11.5)