FastQCFastQC Report
Thu 2 Jun 2016
DRR028371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445734
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25190.17423675447903972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22220.15369355635269005No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19900.085.646111
GTATCAA40000.062.0096051
TCAACGC46200.054.1252024
ATCAACG46850.053.6836783
CAACGCA48350.051.2810825
AACGCAG50150.049.585056
TATCAAC52400.047.3061072
CGCAGAG56300.044.4261058
ACGCAGA56300.044.1685647
TAGATCG1401.41553755E-841.4275745
GCAGAGT68550.035.746779
GTACATG33650.033.838211
TACATGG35600.030.1324272
ACATGGG36500.029.588013
GTACCGA1100.00967411926.3636
TGGTATC9900.025.624492
CATGGGG29800.024.808834
CGTGTCG2051.29102355E-524.7554999
CAGAGTA66750.024.1117769
CTCACCG2552.7090373E-622.744559
Produced by FastQC (version 0.11.5)