FastQCFastQC Report
Thu 2 Jun 2016
DRR028370_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028370_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401363
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40790.2910737617590874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC11750.047.4781844
ATCAACG12450.044.22683
CAACGCA13150.042.9775055
GTACATG30850.038.058931
AACGCAG15200.037.6592256
GTATCAA16800.036.238211
GGTATCA11250.034.1444471
TACATGG32300.033.6541632
CGCAGAG17200.032.863688
ACGCAGA17750.032.2513357
TATCAAC19000.030.8945222
ACATGGG40450.026.8666783
CATGGGG31050.025.9001674
CTTAGCG2155.0686195E-420.2259779
GCAGAGT25400.018.2617769
ATGGGGG28100.017.7916035
GTATAAT14050.017.5387781
ACACCGT3352.873712E-517.3034656
GTCTAAG7301.8189894E-1216.8780691
GAGTACA23700.016.2078061
Produced by FastQC (version 0.11.5)