FastQCFastQC Report
Thu 2 Jun 2016
DRR028370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401363
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20030.1429322737934425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18230.13008763610856003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19150.078.4092941
GTATCAA38200.058.6760831
TCAACGC41000.053.915044
ATCAACG41150.053.8946343
CAACGCA41650.052.5684745
AACGCAG43100.051.304566
TATCAAC47200.047.293632
CGCAGAG49700.043.7621158
ACGCAGA49800.043.6742327
GCAGAGT60900.034.8805859
GCGTGCG850.002742501434.1172879
GTACATG30000.032.4003331
TACATGG31450.030.6496092
ACATGGG33150.027.7660483
TTACGCA1358.1000215E-426.8429454
CATGGGG27900.024.678194
GTGGTAT11300.023.1094741
TGGTATC11400.022.251392
CAGAGTA60050.021.7316599
TAACGCA1750.003666493320.7074154
Produced by FastQC (version 0.11.5)