FastQCFastQC Report
Thu 2 Jun 2016
DRR028369_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028369_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524446
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44340.29085976151336285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16650.047.4293634
ATCAACG18000.044.276123
GTATCAA20600.042.231371
CAACGCA18900.041.402415
GGTATCA13500.040.813231
AACGCAG19500.039.3853656
GTACATG40200.036.4289321
CGCAGAG21900.034.7417538
ACGCAGA22500.034.135117
TACATGG40500.034.003182
TATCAAC25400.033.1013152
ACATGGG46050.028.4772643
CATGGGG37500.027.2409924
GTCGTAC1606.832234E-527.1864451
GCAGAGT30550.022.7746879
ATGGGGG30250.022.51475
ATACGGT2104.3339873E-420.7012026
GAGTACA28350.020.2020381
CGTACAC2205.931794E-419.759593
GTATATA16900.018.8750061
Produced by FastQC (version 0.11.5)