FastQCFastQC Report
Thu 2 Jun 2016
DRR028369_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028369_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524446
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20650.13545904544995363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19030.12483223413620424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20300.081.832841
GTATCAA37600.060.5800971
TCAACGC43950.051.7831424
ATCAACG44100.051.7713663
CAACGCA45300.049.6210985
AACGCAG47850.047.2797856
TATCAAC50100.044.8478472
CGCAGAG53150.041.883028
ACGCAGA53500.041.8800857
GCAGAGT64850.034.2148259
GTACATG37850.033.539791
TACATGG39750.030.0862
ACATGGG40950.029.027363
CATGGGG34500.025.2104034
CAGAGTA62650.024.9997439
ATGGGGG27900.020.011955
GAGTACA27350.019.096921
AGAGTAC57500.018.63657410-14
CCGTGCA2952.1242264E-417.205969
GTATAGA10200.017.0686531
Produced by FastQC (version 0.11.5)