FastQCFastQC Report
Thu 2 Jun 2016
DRR028368_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028368_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008886
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56500.28125040445301525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC20450.052.4384544
ATCAACG20750.050.9831963
CAACGCA22150.049.0705225
AACGCAG23550.045.5380026
GTATCAA25550.042.8274041
ACGCAGA27100.037.7028967
TATCAAC28600.036.9932562
CGCAGAG27400.035.438818
GTACATG45400.035.11581
GGTATCA17000.033.675491
TACATGG49250.031.635012
ACATGGG53050.028.4099393
GCAGAGT39150.023.3247999
CATGGGG48200.023.1502234
GAGTACA33500.019.4685421
GTCTAGC4553.4597178E-919.1119751
GTGTACG2701.08372165E-418.7875421
GTATTAG15850.018.745211
CCGTACA1950.00685735718.5829439
GTCTTAC9550.018.211411
Produced by FastQC (version 0.11.5)