FastQCFastQC Report
Thu 2 Jun 2016
DRR028368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008886
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24300.064.468491
GTATCAA41350.054.548761
ATCAACG42550.051.779813
TCAACGC42650.051.65844
CAACGCA44850.048.979915
AACGCAG46650.047.4008336
TATCAAC51700.043.31662
ACGCAGA53750.040.7348637
CGCAGAG54800.039.689768
GTACATG43700.032.3633271
GCAGAGT70500.029.822689
TACATGG47250.027.7627942
ACATGGG49900.025.9979383
TCGTGCG1550.001809126323.3870859
CATGGGG42600.023.307594
CAGAGTA67450.020.959969
GTATACG2455.1026167E-520.7220151
TCTATAC14950.019.3912243
ATGGGGG27600.018.9130345
AGAGTAC59300.017.45543310-14
Produced by FastQC (version 0.11.5)