FastQCFastQC Report
Thu 2 Jun 2016
DRR028367_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028367_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953697
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58550.29968823210559264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC20700.049.7041854
GTATCAA23150.049.469471
ATCAACG21500.047.8547253
CAACGCA22850.045.9810685
AACGCAG25150.041.4868626
GTACATG50600.039.9654661
TATCAAC28500.037.124542
TACATGG52050.036.199762
ACGCAGA28150.035.781297
GGTATCA15000.035.757871
CGCAGAG28000.035.4572038
ACATGGG60350.030.6152533
CATGGGG48000.028.9824374
GCAGAGT39500.023.85069
GTATAGA13750.023.1942941
CGACGAC5650.021.805992145
ACCGTAC1750.003669544620.7049358
GAGTACA37200.020.068991
ATGGGGG41250.019.6739375
CAGAGTA37450.018.3833589
Produced by FastQC (version 0.11.5)