FastQCFastQC Report
Thu 2 Jun 2016
DRR028367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953697
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22510.11521745695468642No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20590.10538993508205213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21750.081.701251
GTATCAA43200.064.135941
ATCAACG50800.053.7883383
TCAACGC51400.053.160464
CAACGCA54050.050.301335
AACGCAG54650.049.7490776
TATCAAC56500.047.9770622
ACGCAGA63300.042.950827
CGCAGAG64150.042.042668
GTACATG52500.037.715961
TACATGG56800.034.07012
GCAGAGT77400.033.7213869
ACATGGG57200.032.818163
CATGGGG45050.028.7984264
GTACGAC1902.1985754E-422.8880183
CAGAGTA75400.022.1156839
ATGGGGG37500.021.2669565
GTCGTAC1850.00503622319.602891
GAGTACA39200.018.6877561
CTATACG2751.2457688E-418.449134
Produced by FastQC (version 0.11.5)