FastQCFastQC Report
Thu 2 Jun 2016
DRR028366_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028366_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352322
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39850.2946783384430631No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG47250.054.307211
GGTATCA12150.051.903781
TCAACGC19550.050.039634
GTATCAA21350.049.908611
ATCAACG19850.048.9201133
TACATGG47050.048.368382
CAACGCA20900.046.113965
AACGCAG21850.043.115666
TATCAAC25350.039.1682472
CATGGGG44750.035.463224
ACCGCGT1251.2689845E-534.7856838
ACGCAGA25900.034.136537
ACATGGG62100.033.7248043
CGCAGAG27400.031.2097848
TACATGA10150.026.419662
GCAGAGT31300.024.775999
GAGTACA30700.024.0834031
ATGGGGG41400.021.7044325
ACCGAGT1700.003100735621.3147588
ATGGGGA22100.020.9852815
Produced by FastQC (version 0.11.5)