FastQCFastQC Report
Thu 2 Jun 2016
DRR028366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352322
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21730.160686582041851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18910.1398335603502716No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20250.084.155291
GTATCAA43300.071.679421
ATCAACG51800.059.466573
TCAACGC51950.059.155354
CAACGCA54900.055.7332575
TATCAAC56850.053.9291572
AACGCAG57250.053.3188746
ACGCAGA64900.046.8105477
CGCAGAG65400.046.5635268
GTACATG45800.041.8000761
GCAGAGT73550.040.910979
TACATGG45900.039.7925342
ACATGGG50400.035.2329753
TGGTATC9200.032.3005372
CATGGGG42450.032.0991744
GTGGTAT10500.031.0786271
GTACGGA1255.152265E-429.0024266
CAGAGTA70500.023.654469
ATGGGGG35000.023.201945
TATACCG1700.003091849421.3253155
Produced by FastQC (version 0.11.5)