FastQCFastQC Report
Thu 2 Jun 2016
DRR028365_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028365_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992991
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57480.2884107354222874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC20800.050.8585664
ATCAACG20550.050.772123
GTATCAA24200.046.1292951
CAACGCA23250.045.5038645
AACGCAG24700.042.244776
GTACATG47300.038.466621
TATCAAC29000.036.9868772
GGTATCA15500.035.542941
ACGCAGA30500.034.2147877
TACATGG50650.033.9119572
CGCAGAG30150.033.8917438
ACATGGG54800.029.6170713
CATGGGG45800.025.3121574
GCAGAGT41350.024.0138539
GAGTACA34250.022.2228741
TATACCG2256.89437E-419.3235575
CGACGAC6101.8189894E-1219.010607145
GTATTAT17300.018.4364811
AGTACAT39100.018.1649112
ATAGCAC10150.017.8463153
Produced by FastQC (version 0.11.5)