FastQCFastQC Report
Thu 2 Jun 2016
DRR028365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992991
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22740.11409986296977757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.072.3822561
GTATCAA45900.055.7796751
TCAACGC48000.052.093874
ATCAACG47850.051.954233
CAACGCA50600.049.1540645
AACGCAG53550.046.581646
TATCAAC56150.044.9198462
CGCAGAG60250.040.559138
ACGCAGA61200.040.403487
GTACATG46150.035.3610151
GCAGAGT72800.032.7702949
TACATGG49250.031.7874932
ACATGGG49900.030.5019423
CATGGGG40300.030.0344834
CGTACAC1902.1986582E-422.8879243
GAGTACA35150.020.221561
CAGAGTA69050.019.7428559
ATGGGGG30500.019.2575475
GTGGTAT16600.017.9138831
GTCGTAC2050.0091082217.690091
Produced by FastQC (version 0.11.5)