FastQCFastQC Report
Thu 2 Jun 2016
DRR028364_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028364_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498230
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43710.2917442582247051No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17750.053.8833544
ATCAACG18450.052.229973
GTATCAA21200.049.912871
CAACGCA20200.046.635295
GGTATCA13400.045.9737171
AACGCAG21450.045.2704436
GTACATG41650.043.15511
TATCAAC25400.039.3768422
ACGCAGA24200.039.2316747
TACATGG44850.036.8440932
CGCAGAG24750.035.725658
ACATGGG54600.028.7959613
CATGGGG41950.027.6354834
GCAGAGT32500.024.7550749
GAGTACA31700.024.463581
AGTACAT32600.023.1212462
TAATACG2404.384837E-521.1332264
ATGGGGG35000.021.1181015
GTATTAG12250.017.7492771
CAGAGTA28400.017.3546249
Produced by FastQC (version 0.11.5)