FastQCFastQC Report
Thu 2 Jun 2016
DRR028364_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028364_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498230
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24530.16372653063948792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22950.1531807532888809No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22850.083.167591
GTATCAA44950.059.866411
TCAACGC50850.052.3130044
ATCAACG51450.051.7029383
CAACGCA52850.049.655875
AACGCAG55200.047.5418976
TATCAAC58100.046.15942
ACGCAGA61800.042.3472987
CGCAGAG62200.041.958428
GTACATG42350.036.8234141
GCAGAGT73450.034.7422459
TACATGG44550.033.3534432
ACATGGG47250.030.9873263
GTGGTAT11900.029.866761
TAGACCG1000.00607752928.9979295
TGGTATC12400.028.6423682
CATGGGG39550.027.6735574
TAGCGCA1450.001227239124.9982159
CAGAGTA71900.023.1902779
TATACGG2404.3671484E-521.1443235
Produced by FastQC (version 0.11.5)