FastQCFastQC Report
Thu 2 Jun 2016
DRR028363_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028363_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518640
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43900.2890744350208081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG16100.052.2035523
TCAACGC16250.051.7199634
CAACGCA17000.049.4431045
GTATCAA19550.045.6020361
AACGCAG19350.042.316386
TATCAAC22700.039.2688062
GTACATG29450.037.9019281
ACGCAGA20350.037.7481357
CGCAGAG20450.037.211648
GGTATCA11800.036.854891
TACATGG31650.033.4308622
ACATGGG37650.025.2100813
GTATACG2401.6025824E-624.1604291
GCAGAGT28900.023.0728659
CATGGGG31900.020.8954014
GAGTACA23200.019.9948371
GTATTAG11650.019.9090211
GTATAAG9600.018.1203211
TAATACT17900.015.7857484
AGTACAT26450.015.617722
Produced by FastQC (version 0.11.5)