FastQCFastQC Report
Thu 2 Jun 2016
DRR028363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518640
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25420.1673866090712743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21900.1442079755570774No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.074.256351
GTATCAA38900.068.245271
ATCAACG43950.058.7087943
TCAACGC44700.057.723754
CAACGCA46250.055.020165
AACGCAG48700.052.5499426
TATCAAC49700.052.4998632
ACGCAGA54000.047.3922587
CGCAGAG53700.047.387018
GCAGAGT64750.038.5163549
GTACATG33650.034.273081
ACATGGG36350.030.506973
TACATGG37450.030.1915132
CATGGGG29950.025.6519934
CAGAGTA63750.021.9484379
ATACCGT2653.7829232E-621.8862116
CTAGTAC4751.2732926E-1121.3622263
GAGTACA25250.020.9701981
TATACTG13750.020.563095
GTGGTAT11500.020.1833481
Produced by FastQC (version 0.11.5)