FastQCFastQC Report
Thu 2 Jun 2016
DRR028362_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028362_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1442538
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42570.29510487765313637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG15250.057.9633
TCAACGC15150.057.869374
CAACGCA16700.052.9376535
GTATCAA19550.051.1705361
AACGCAG18100.048.8413286
TATCAAC21750.042.987532
ACGCAGA20100.042.9074487
GTACATG34800.041.453521
CGCAGAG20750.041.2140928
TACATGG37900.034.9964452
GGTATCA10850.034.7425161
ACATGGG43100.028.5780473
GCAGAGT28500.027.71819
ATACCGG1450.001230526224.986666
CATGGGG36850.023.9882374
GTATATA16400.021.2170521
AATACCG2505.999813E-520.2898755
TAATCGT2155.0782243E-420.2203564
GAGTACA25600.020.105091
TATATCG2205.924962E-419.7628655
Produced by FastQC (version 0.11.5)