FastQCFastQC Report
Thu 2 Jun 2016
DRR028362_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028362_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1442538
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26350.18266416551938322No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22300.15458864861792201No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19550.081.251811
GTATCAA38300.063.4427031
TCAACGC44400.053.868564
ATCAACG44350.053.7658653
CAACGCA45900.051.655925
TATCAAC47950.050.182682
AACGCAG48000.049.6980936
ACGCAGA53450.044.4949957
CGCAGAG54350.043.8915988
GCAGAGT65900.035.6487779
GTACATG34050.032.3789021
TACATGG36350.029.7088782
ACATGGG39500.027.5231743
GTACGAC1100.009687494526.3555263
CATGGGG34750.024.1939934
TATATCG1500.0014936524.1692855
GGGGTCG1952.613605E-422.3101089
CAGAGTA61650.022.2286859
CTGTCGC4901.8189894E-1120.716539
TGTACGA1750.003666035420.7079122
Produced by FastQC (version 0.11.5)