FastQCFastQC Report
Thu 2 Jun 2016
DRR028361_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028361_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682600
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49560.29454415785094495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG19300.047.3052183
TCAACGC19250.047.0516744
GTATCAA21950.044.5810781
CAACGCA21450.042.227125
GTACATG43000.040.9627531
GGTATCA14650.039.582541
TATCAAC25050.037.8998152
AACGCAG24100.037.88236
TACATGG45600.036.0773432
ACGCAGA28050.031.2580057
CGCAGAG26900.030.7086938
ACATGGG56700.028.1148263
CATGGGG45450.026.9431654
GAGTACA30750.023.5725291
GCAGAGT36000.021.739179
GCACCGT3157.329072E-720.7021186
GTATTAG14400.019.6314961
TCTAGCG1900.0059024119.0717242
TCGCCGG5407.4578566E-1118.788057145
GTATAAG9700.018.6818371
Produced by FastQC (version 0.11.5)