FastQCFastQC Report
Thu 2 Jun 2016
DRR028361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682600
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23590.1401996909544752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20610.12248900511113753No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24300.072.5330661
GTATCAA42950.061.6404461
TCAACGC49350.053.3129584
ATCAACG50050.052.7121353
CAACGCA51050.051.1192635
TATCAAC54750.048.3194542
AACGCAG55000.047.8433726
CGCAGAG61700.042.2955978
ACGCAGA62400.041.9372987
GCAGAGT74700.034.0615469
GTACATG46400.030.3263281
TACATGG49400.027.8766122
ACATGGG49950.026.408833
GTGGTAT12500.026.1119061
CAGAGTA73000.022.8393029
CATGGGG43950.021.2737544
GAGTACA35600.020.7819481
TGGTATC13400.020.5537992
GTATACG2205.8875454E-419.7817461
TACCGAG2205.911174E-419.7699897
Produced by FastQC (version 0.11.5)