FastQCFastQC Report
Thu 2 Jun 2016
DRR028360_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028360_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658476
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49120.2961755250000603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17850.047.087684
ATCAACG18150.046.3093723
GTATCAA21700.044.7586751
CAACGCA19150.043.8911255
AACGCAG20450.041.4565546
GGTATCA14350.038.892961
ACGCAGA22650.037.11677
GTACATG34750.036.9190561
TATCAAC25250.036.7379532
CGCAGAG22900.035.12918
TACATGG40550.029.4889622
ACATGGG42750.026.1018983
CATGGGG36800.025.202854
GCAGAGT33800.021.0136789
ATGGGGG27600.019.4271975
TAGATCG1950.00686521118.5790255
GAGTACA28000.018.3794481
CTACGAC2450.00122209917.7448643
TATACTG14800.017.1353865
CAGAGTA30450.016.661099
Produced by FastQC (version 0.11.5)