FastQCFastQC Report
Thu 2 Jun 2016
DRR028360_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028360_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658476
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38730.23352764827468112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32020.19306881739621196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18700.11275411884163535No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25250.083.608031
GTATCAA51050.062.2436071
TCAACGC55600.056.3143734
ATCAACG56900.055.1551323
CAACGCA57050.053.7493975
AACGCAG59100.052.0076566
TATCAAC61300.051.314432
ACGCAGA67400.045.388047
CGCAGAG67400.045.2804838
AACGCCG1307.363269E-944.6103366
GCAGAGT82450.036.311849
ACGCCGA1902.0800508E-730.5228637
GTACATG34200.029.4852681
GCGCGCA1504.403828E-528.996729
CGCCGAG2003.2597563E-728.996728
CAACGCC2104.9947266E-727.6159255
TACATGG38250.025.5807132
ACATGGG38050.024.9532383
GTGGTAT13350.023.9104521
CAGAGTA77400.022.7590529
Produced by FastQC (version 0.11.5)