FastQCFastQC Report
Thu 2 Jun 2016
DRR028359_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028359_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066956
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60520.2927977179968998No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG24150.047.1080673
TCAACGC24300.046.521344
CAACGCA25200.045.434995
GGTATCA18750.044.0667841
GTATCAA31050.040.148991
AACGCAG28200.039.8305666
GTACATG50750.035.132321
ACGCAGA31500.034.0483137
CGCAGAG31350.033.9808928
TATCAAC34950.033.383642
TACATGG52400.031.947412
ACATGGG54100.029.868983
CATGGGG47500.024.8672564
GCAGAGT44700.021.4008949
GAGTACA37450.018.5792061
ACCGCGT2050.00915177517.6754768
GTACCGT2050.00915433717.6746186
GTATATA25300.017.188521
GTATAAC9350.017.0537091
TACCGTA2650.002064899816.407787
Produced by FastQC (version 0.11.5)