FastQCFastQC Report
Thu 2 Jun 2016
DRR028359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066956
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46680.22583935023290286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36510.17663656120401208No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21270.10290494814596925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32500.083.0165941
GTATCAA58900.060.2141951
ATCAACG68800.050.6695063
TCAACGC69150.050.4130444
CAACGCA71450.048.4055675
AACGCAG74700.046.2025036
TATCAAC76100.045.90422
CGCAGAG85100.040.5561378
ACGCAGA85700.040.2721987
GCAGAGT100950.033.4702389
GTACATG48900.030.7020111
TATACCG1902.0766856E-730.529215
TACATGG50650.027.7595442
ACATGGG50000.027.2507133
CATGGGG43450.024.1862344
CAGAGTA95300.023.8139069
TAGACCG1600.002174731322.6583985
GTGGTAT15100.020.653651
TAATACG4152.503657E-819.210324
TAATCCG2359.1987435E-418.5123925
Produced by FastQC (version 0.11.5)