FastQCFastQC Report
Thu 2 Jun 2016
DRR028358_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028358_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592494
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22430.3785692344563827No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17490.2951928627125338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8550.087.3226851
GTATCAA19150.069.647421
ATCAACG23300.054.113063
TCAACGC23750.053.392864
CAACGCA24350.052.383695
GTACATG50600.049.565741
TACATGG47750.049.4796452
TATCAAC27150.048.8498952
AACGCAG26300.047.3974656
GATGACC2150.047.1963849
CGCAGAG27900.042.8646138
ACGCAGA29200.042.197367
GCAGAGT26900.041.224789
CATGGGA23800.041.1022454
TGGTATC3050.040.3953552
TACATGA3150.039.1129652
GTGGACC2600.039.017863
GCTCTAG1502.600791E-838.6593671
CATGGGG29950.037.9849554
GTGGTAT3650.037.73261
Produced by FastQC (version 0.11.5)