FastQCFastQC Report
Thu 2 Jun 2016
DRR028358_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028358_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592494
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61741.0420358687176579No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49090.8285315969444417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30370.5125790303361721No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8340.1407609190979149No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7830.13215323699480502No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC7610.12844011922483603No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT6400.10801797149000665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6390.10784919340955351No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG6380.10768041532910039No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.0121.047991
GTATCAA50300.077.538231
GTGGTAT3450.063.038191
TGGTATC3650.059.568962
ATCAACG66550.058.8081253
TCAACGC67850.057.467734
CAACGCA68650.056.4043125
TATCAAC69100.056.3232732
AACGCAG72150.054.0701456
TCTGTCG1750.053.866278
ACGCAGA80300.048.582337
CGCAGAG80200.048.4620748
GTCTTAC951.9206564E-645.7856371
GCAGAGT84600.045.427329
TAAACGC803.7402366E-545.297234
TACATGG46950.043.8403852
GTACATG47250.043.726491
CATGGGA22250.042.671014
GATGACC2750.039.5521559
ACATGGG51500.039.2634623
Produced by FastQC (version 0.11.5)