FastQCFastQC Report
Thu 2 Jun 2016
DRR028357_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028357_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707824
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49680.29089648582055294No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22150.051.357084
ATCAACG23550.049.2255633
CAACGCA24000.047.7058075
GTATCAA26200.046.7547531
GGTATCA14750.045.7015841
AACGCAG25700.044.830826
GTACATG47600.037.460061
ACGCAGA30500.036.8273167
TATCAAC32000.036.4608572
CGCAGAG30800.035.527488
TACATGG48900.034.3819162
ACATGGG54300.030.956353
CATGGGG43950.027.8613534
GCAGAGT38300.025.9237779
GAGTACA35150.021.8585261
CAGAGTA35300.020.32539
GTATAAG12100.019.169241
AGTACAT40550.018.2288912
ATGGGGG36150.017.0386835
TATAGGG9050.016.815962
Produced by FastQC (version 0.11.5)