FastQCFastQC Report
Thu 2 Jun 2016
DRR028357_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028357_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707824
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29690.1738469537844649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25420.14884437740657117No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25450.075.8027951
GTATCAA48050.061.1298071
TCAACGC53350.054.201574
ATCAACG54400.053.022183
CAACGCA56650.050.2957575
AACGCAG58500.048.953076
TATCAAC62150.046.5283742
ACGCAGA65500.043.2786947
CGCAGAG66950.042.341378
GCAGAGT78900.035.4690069
GTACATG49800.034.5151981
TACATGG49450.032.976332
ACATGGG53000.030.903383
CATGGGG42550.026.2297744
CAGAGTA77400.023.6983599
GTGGTAT11200.021.3691521
CATGGGA21300.020.4147974
GAGTACA37650.019.455741
GTATACG2751.2394924E-418.4610631
AGAGTAC72700.017.87790710-14
Produced by FastQC (version 0.11.5)