FastQCFastQC Report
Thu 2 Jun 2016
DRR028356_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028356_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14002
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2661.8997286101985429No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA1050.7498928724467933No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA690.4927867447507499No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA650.46421939722896727No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC540.38565919154406514No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA440.3142408227396086No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN400.28567347521782605No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT340.2428224539351521No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT320.22853878017426082No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA290.20711326953292386No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC280.19997143265247821No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG270.19282959577203257No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA250.17854592201114128No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA240.1714040851306956No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG230.16426224825024996No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG230.16426224825024996No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.16426224825024996No Hit
ACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGA220.1571204113698043No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT220.1571204113698043No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC220.1571204113698043No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT220.1571204113698043No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT220.1571204113698043No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC200.14283673760891302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.14283673760891302No Hit
ATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATC200.14283673760891302No Hit
GTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATA190.13569490072846735No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG190.13569490072846735No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA180.1285530638480217No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA180.1285530638480217No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA180.1285530638480217No Hit
ATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCATCACCA180.1285530638480217No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.12141122696757604No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG170.12141122696757604No Hit
GCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTG170.12141122696757604No Hit
ATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATT160.11426939008713041No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.11426939008713041No Hit
CATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG160.11426939008713041No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC150.10712755320668477No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA150.10712755320668477No Hit
GCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCG150.10712755320668477No Hit
GCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATC150.10712755320668477No Hit
GGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGAACGATCG150.10712755320668477No Hit
ATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTA150.10712755320668477No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC150.10712755320668477No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC150.10712755320668477No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCAC100.0070316778144.896065
TTAATCA100.0070316778144.896064
TGCTTCG501.9489153E-457.9584248
GAACATG400.00594389954.336021
GTATCAA701.6174943E-551.7485921
CTAATGC752.4328312E-548.2986874
CGCAGAG752.4328312E-548.2986878
GCTTCGT604.795135E-448.2986879
GGTATCA450.00946939548.2986871
TAATGCT803.562659E-545.2800185
CGCCTAA657.1148016E-444.58341
CCTAATG657.1148016E-444.58343
ATGCTTC657.1148016E-444.58347
TATCAAC1002.5571626E-643.468821
AAGGCCG1303.3807555E-739.0104755
AACAACA750.001438818838.6389473
GCCTAAT750.001438818838.6389472
CAACACA750.001438818838.6389475
TCAACGC959.818557E-538.1305433
ATCAACG959.818557E-538.1305432
Produced by FastQC (version 0.11.5)