FastQCFastQC Report
Thu 2 Jun 2016
DRR028355_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028355_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105696
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN62980.29909350637508925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG20000.051.805473
TCAACGC20000.051.0796974
CAACGCA22200.046.6715935
GTATCAA26500.044.8626561
AACGCAG23700.043.7187356
TATCAAC30050.038.1053282
GTACATG47900.035.8673971
ACGCAGA28600.035.7243277
CGCAGAG28100.035.329348
GGTATCA16300.031.1313131
TACATGG51150.030.8875832
CATGGGG46450.026.3608954
ACATGGG57400.026.0030863
GAGTACA35100.022.0985431
GCAGAGT40500.021.1154379
TCTAACG3001.1123882E-519.3213863
ATGGGGG35850.018.3916975
TATACTG19900.017.8407275
ATTATAC19200.017.3590563
AGTACAT41100.017.280542
Produced by FastQC (version 0.11.5)